Journal:

Article Title: Performance of Commercially Available Enzyme Immunoassays for Detection of Antibodies against Herpes Simplex Virus Type 2 in African Populations

doi: 10.1128/JCM.42.7.2961-2965.2004

Figure Lengend Snippet: Initial selection of HSV-2-specific serological tests

Article Snippet: In 2001, three tests were approved by the U.S. Food and Drug Administration (FDA) for the diagnosis of HSV-2 infection, including the HerpeSelect (Focus Technologies, Inc., Cypress, Calif.), the HSV-1 and HSV-2 IgG differentiation immunoblot (Focus Technologies), and the POCkit HSV-2 (Diagnology, Ltd., Belfast, Northern Ireland).

Techniques: Selection, Purification, Enzyme-linked Immunosorbent Assay, Recombinant

Journal: bioRxiv

Article Title: Base Pairing and Functional Insights into N 3 -methylcytidine (m 3 C) in RNA

doi: 10.1101/2020.08.27.270322

Figure Lengend Snippet: Fluorescent ( A and B ) gel images of standing-start primer extension reactions for HIV-1 RT indicated using m 3 C containing RNA template and the corresponding natural template. Lanes: L, ladder; P, primer; Nat, natural template with all four dNTPs; A, T, G, and C, reactions in the presence of the respective dNTP; N, reactions in the presence of all four dNTPs.

Article Snippet: RT assays were performed with AMV RT (ThermoFisher), HIV-1 RT (AS ONE Corp.), MMLV RT (ThermoFisher) and MutiScribe™ RT (ThermoFisher) in 20 μL total solution containing 10X reverse transcription buffer: 50 mM Tris (pH 8.3), 75 mM KCl, 3 mM MgCl 2 , 10 mM DTT.

Techniques:

Journal:

Article Title: Preclinical and Clinical Performance of the Efoora Test, a Rapid Test for Detection of Human Immunodeficiency Virus-Specific Antibodies

doi: 10.1128/JCM.43.5.2399-2406.2005

Figure Lengend Snippet: Efoora HIV rapid test preclinical testing locations and information

Article Snippet: Specimens tested at sites 2 and 4 were predominantly, if not exclusively, from patients whose infection originated in the United States and thus were probably subtype B. table ft1 table-wrap mode="anchored" t5 TABLE 2. caption a7 Site Date No. of specimens tested Reference test(s) a Specimen type(s) U.S. East Coast March 1999 1,931 Genetic Systems HIV-1/2, Bio-Rad WB Serum February 2000 291 Genetic Systems HIV-1/2, Genetic Systems rLAV EIA, Bio-Rad WB Fresh whole blood February 2001 528 Genetic Systems HIV-1/2, Bio-Rad WB Serum, plasma, whole blood U.S. Midwest June 1999 1,076 Genetic Systems HIV-1/2, Abbott HIV-1/2, Bio-Rad WB Serum, plasma Ivory Coast, West Africa June 1999 962 Enzygnost Plus, immunocapture HIV Serum, plasma South Africa March 2000 150 Genetic Systems HIV-1/2 Serum, plasma, whole blood Open in a separate window a rLAV, recombinant lymphadenopathy-associated virus (HIV-1).

Techniques: